Background image
Home Synthetic DNA Why Cell-Free DNA

Why Synthetic, Cell-Free DNA?

As programs scale, the limitations of traditional plasmid-based production, including long timelines, bacterial-derived impurities, and complex scale-up, are now a bottleneck for speed, scalability, and consistency.

By eliminating bacterial processes, synthetic DNA can be produced in as little as two weeks (GMP and RUO) rather than months, enabling significantly faster timelines while improving process control and reducing variability. 

Engineered DNA Is the Foundation for Every Major Modality in Modern Biologics

Artis_Asset-3C

Cell and Gene Therapy

CAR-T & CAR-NK

Engineering Cell Therapies

Viral Vectors: AAV & LVV

Artis_Asset-4C

RNA and DNA Therapeutics / Vaccines

IVT DNA Templates 

Regulatory Elements

Synthetic DNA Constructs

Artis_Asset-2B

Genome Engineering Programs

Gene Editing

Base Editing

Transgenes

Artis_Asset-1C

Synthetic Biology and Metabolic Engineering

Metabolic Pathways

Gene Circuits

Bioproduction

 

 

Traditional plasmid DNA production adds complexity to programs

For the past 30+ years, DNA has been manufactured by producing plasmids in bioreactors using E. coli as the host, often resulting in costly bottlenecks in workflows. Completely removing the reliance on bacterial fermentation, synthetic methods use advanced chemistry or enzymatic processes to rapidly generate high-purity DNA.

oDNA Outperforms pDNA in Customer Programs

Across applications oDNA accelerates, scales, and performs better than comparable plasmid DNA. In over 30 customer programs, with GOI lengths of 1-15.6 kb, and polyA tails from 40-135 bp, oDNA is the superior starting material. 

94%

saw higher IVT yield

71%

saw improved mRNA integrity 

86%

saw reduced dsRNA

100%

saw capping or circularization efficiency 

100%

saw mRNA functionality

Want to learn more?
Contact Us